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IgG Fc Glycopeptides by LC-MS Immunoglobulin G antibodies

HeLa HCP ELISA Kit

Product name: Hela human whole cell lysate

SKU: LISADO0001

Description

This cell lysate is prepared from human Hela cells using Boster’s RIPA Lysis Buffer using a standard complete cell lysate protocol. The concentration was determined using the BCA assay procedure and then diluted using dithiothreitol (DTT) and reducing SDS sample loading buffer, heated for 5 minutes at 100°C.

Expression Profiling:

Read about HeLa cell gene expression and protein transcription profiling details: The genomic and transcriptomic landscape of a HeLa HCP Elisa Kit.

Applications

Western blot control (20-40 μg/lane)

Quality control

  • The isolated protein pattern on SDS-PAGE gel is visualized by Coomassie blue staining. The pattern is consistent with each batch.
  • The isolated protein is analyzed by Western Blot using a GAPDH or beta-actin antibody. The level of expression is consistent with each lot.
  • This lysate is used to test for many of Boster’s primary antibodies. See the quality control images of these antibodies for more details.

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Properties

Form: Liquid

Size: 200μg

Concentration: 2μg/μl

Formulation: Cell lysate is in SDS-PAGE sample buffer, pH 6.8, containing 50 mM TRIS-HCl, 2% SDS, 100 mM DTT, 10% glycerol, and 0.01% bromophenol blue.

Storage

Shipped at 4˚C. Aliquot and store at -20˚C or -80˚C at time of delivery. Avoid repeated freezing and thawing.

Background

HeLa cells are human epithelial cells from a fatal cervical carcinoma. The cell line was derived from cervical cancer cells taken from Henrietta Lacks, a patient who died of her cancer on October 4, 1951. Horizontal gene transfer of human papillomavirus 18 (HPV18) into cervical cells humans created the HeLa genome, which is different from both parent genomes in various ways, including the number of chromosomes.

The cell line was to be significantly long-lived and prolific and has since been widely used in scientific research. HeLa cells have a modal chromosome number of 82, with 4 copies of chromosome 12 and 3 copies of chromosomes 6, 8, and 17. HeLa cells are adherent cells and maintain contact inhibition in vitro.

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